Abstract: A 2D augmented reality environment with the characteristics of microscope image is implemented for a microoperation robot system in this paper. Generating micromanipulator, kinematics modeling for micro manipulator and micro image processing for both micro manipulator and microoperating object are the main problems in the implementing process. After the key algorithms for these problems have been successfully carried out, the virtual microoperation environment is implemented in this paper. The environment can be effectively used to preview or rehearse the real micro operation and do path planning for micromanipulator and train the operators.