Abstract: A magnetotactic bacterial microrobot system is fabricated using magnetotactic bacteria with magnetic responsiveness.Firstly, a dynamic model of magnetotactic bacteria MO-1 is established and the dynamic features of MO-1 cells in different magnetic fields are analyzed through simulation.The simulation results agree with the experimental ones.Then MO-1 cells are coated by rabbit anti-MO-1 polyclonal antibodies to construct MO-1 bacterial microrobots.The microrobots can bind Staphylococcus aureus based on the affinity of Fc fragment of antibody.Staphylococcus aureus can be captured and separated in microfluidic chip by MO-1 microrobots through a fabricated magnetic controlling system.The experimental results show that the magnetotactic bacterial microrobot system constructed here can achieve the separation of Staphylococcus aureus effectively, suggesting its important role in medical detection and analysis.