Abstract: A navigation system for non-invasive rat robot is described here that can control the rat navigation via ultrasonic, electrical epidermal and LED(light-emitting diode) photic stimulators.It receives commands from a remote host computer to remotely deliver ultrasonic,electrical epidermal and LED photic stimuli to the auditory,pain and visual senses of the rat respectively,and thus its motion control is accomplished.The experiments demonstrate that the appropriate coordination of the three stimuli enables the rat to proceed and make right and left turns effectively and non-invasively,what's more,the telemetry camera mounted on the rat head also achieves remote image acquisition and helps to adjust rat navigation path over a distance of 300 m.